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Table 1 Growth of Coxiella burnetii NMI and NMII after UVC exposure

From: Ultraviolet C inactivation of Coxiella burnetii for production of a structurally preserved whole cell vaccine antigen

C. burnetii isolate

UVC Intensity (µW/cm2)

Exposure time (min)

C. burnetii (GE/ml (SD))1

Log difference2 day0/day7

day 0

day 7

NMII

1500

60

7.03E + 04 (8.77E + 04)

2.01E + 03 (8.48E + 02)

-1.54

1000

60

2.54E + 06 (2.08E + 06)

1.01E + 05 (1.62E + 05)

-1.40

15

5.08E + 06 (1.01E + 06)

1.90E + 05 (9.11E + 04)

-1.43

500

60

1.09E + 07 (3.80E + 06)

1.29E + 05 (2.56E + 04)

-1.93

45

2.22E + 07 (5.99E + 06)

3.77E + 05 (1.60E + 05)

-1.77

30

2.74E + 07 (9.48E + 06)

9.83E + 05 (3.27E + 05)

-1.45

15

3.80E + 07 (6.07E + 06)

3.25E + 06 (1.96E + 06)

-1.07

250

15

7.23e + 05 (4.25e + 05)

2.05e + 05 (1.17e + 04)

-0.55

100

20

2.24E + 06 (7.34E + 05)

9.48E + 05 (3.36E + 05)

-0.37

15

5.85E + 05 (2.17E + 05)

1.77E + 06 (4.10E + 05)

0.48

10

3.34E + 06 (4.03E + 05)

5,97E + 07 (8.87E + 06)

1.25

5

3.27E + 06 (1.51E + 06)

1.76E + 08 (5.89E + 07)

1.73

NMI

250

15

1.57E + 08 (4.56E + 07)

2.72E + 07 (5.41E + 06)

-0.76

10

4.73E + 08 (7.18E + 07)

3.43E + 07 (2.79E + 06)

-1.14

5

1,80E + 08 (2,95E + 07)

3,45E + 07 (9,33E + 06)

-0.72

100

20

6.27E + 05 (1.93E + 05)

1.17E + 07 (2.30E + 07)

1.27

15

8.76E + 05 (1.64E + 05)

2.60E + 07 (1.36E + 07)

1.47

10

1.14E + 06 (1.26E + 05)

8.76E + 07 (1.56E + 07)

1.89

5

2.82E + 08 (8.51E + 07)

4.88E + 08 (9.23E + 07)

0.24

  1. 1For recovery, bacteria (1 × 1010 GE/ml) were diluted in ACCM-2 after UVC treatment (day 0) and incubated for 7 days
  2. 2Growth or inactivation was determined as log difference of qPCR quantified bacteria (GE/ml) after incubation. Results represent the average of three independent experiments