Fig. 6

Ultrathin section of C. burnetii NMII after UVC treatment. C. burnetii NMII (A) 24 h and (B) 48 h after UVC treatment at 100 µW/cm² for 20 min and untreated controls (C) after 24 h and (D) 48 h. Swelling and degradation of Coxiella is prominent 24 and 48 h after UVC treatment (A, B). Thin bacteria with coarsely granular electron lucent cytoplasm (arrowheads, examples) were interpreted as swollen SCV. Different degrees of swelling occurs in bacteria where the bacterial wall encloses lysed cytoplasm ( ↑ = mild, ↑↑ = moderate, ↑↑↑ = severe). Bacterial walls are occasionally fragmented (open arrows). LCV (L, examples) and SCV (S, examples) without visible damage are present in the untreated controls (C, D). Bars = 500 nm