Fig. 5

Western Blotting analysis. Representative pictures of the results (A). Blots densitometric analysis and targets quantification (B). Protein samples were obtained by CaCo2 cells cultured in contact with 1:10, 1:20, or 1:50 cell-to-HK L. monocytogenes ratios, or with 1:10, 1:20, or 1:50 cell-to-HK L. innocua ratios, for 30 or 60 min. Samples of HK L. monocytogenes and L. innocua were loaded as negative control, too. Results are normalized to the level of total protein of the studied targets. Data show results of at least three different experiments; results are expressed as relative fold change in comparison to the control ± standard error. Statistical significance is compared to CTR. * p < 0.05; ** p < 0.01; *** p < 0.001; one-way ANOVA, followed by Dunnett’s Multiple Comparison Test. Full-length blots are presented in Supplementary Figure S8. HK Lm: heat-killed L. monocytogenes; HK Li: heat-killed L. innocua