Fig. 3

Effects of HK L. monocytogenes on CaCo2 cell proliferation in anchorage-dependent conditions. CaCo2 cells were cultured in contact with 1:10, 1:20, or 1:50 cell-to-HK L. monocytogenes ratios, or with 1:10, 1:20, or 1:50 cell-to-L. innocua ratios, for 48 h. Representative pictures of results (A). Cell viability was assessed through cell count by trypan blue exclusion assay (B) and MTS assay (C). Data show results of at least three different experiments; results are expressed as the relative number of cells (vs. CTR) ± standard error. Statistical significance is compared to CTR. *** p < 0.001; one-way ANOVA, followed by Dunnett’s Multiple Comparison Test. HK Lm: heat-killed L. monocytogenes; HK Li: heat-killed L. innocua