Fig. 2

Effects of HK L. monocytogenes on JB6 P + cell transformation and tumorigenic capacity in anchorage-independent conditions. Cells were cultured in contact with 1:10, 1:20, or 1:50 cell-to-HK L. monocytogenes ratios, or with 1:10, 1:20, or 1:50 cell-to-HK L. innocua ratios, in soft agar for 31 days. Representative stereoscope images of 3D colony formation (A); number of colonies formed, after crystal violet staining and stereoscope counting. Data show results of at least three different experiments; results are expressed as the total number of colonies ± standard error (B). Statistical significance is compared to CTR. *** p < 0.001; one-way ANOVA, followed by Dunnett’s Multiple Comparison Test. TPA: tumor promoter; HK Lm: heat-killed L. monocytogenes; HK Li: heat-killed L. innocua