Fig. 1

Effects of HK L. monocytogenes on JB6 P + cell transformation and proliferation in anchorage-dependent conditions. Cells were cultured in contact with 1:10, 1:20, or 1:50 cell-to-HK L. monocytogenes ratios, or with 1:10, 1:20, or 1:50 cell-to-HK L. innocua ratios, for 72 h. Representative pictures of the results (A). Cell viability was assessed through cell count by trypan blue exclusion assay (B) and MTS assay (C). Data show results of at least three different experiments; results are expressed as the total number of cells ± standard error. Statistical significance is compared to CTR. *** p < 0.001; one-way ANOVA, followed by Dunnett’s Multiple Comparison Test. TPA: tumor promoter; HK Lm: heat-killed L. monocytogenes; HK Li: heat-killed L. innocua