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Table 1 Purification steps of ZEA-degrading proteins

From: Biotransformation of zearalenone to non-estrogenic compounds with two novel recombinant lactonases from Gliocladium

Name

Purification

steps

Total protein (µg)

Total activity

(U)

Specific activity

(U/mg)

Purification

(-fold)

Yield

(%)

ZHDR52

Crude

24.01

0.791

32.93

1.0

100.0

Purified

1.47

0.288

196.11

6.0

36.4

ZHDP83

Crude

22.34

0.814

36.43

1.0

100.0

Purified

1.24

0.284

229.64

6.3

34.9

ZHD101

Crude

26.55

0.487

18.36

1.0

100.0

Purified

1.61

0.218

135.40

7.4

44.8