Structure predictions and functional insights into Amidase_3 domain containing N-acetylmuramyl-L-alanine amidases from Deinococcus indicus DR1

Table 1 Strains and plasmids used in this study

	Relevant genotype and description	Sources/References
Plasmids
pBAD18	Kan^R, DH5α containing empty pBAD18	[68]
pET28b	protein expression vector, Kan^R	Laboratory strain collection
pMD01	pBAD18 carrying ami1_Di, Kan^R	This work
pMD02	pBAD18 carrying ami1_Di^(1–155a), Kan^R	This work
pMD03	pBAD18 carrying ami1_Di^(H161A), Kan^R	This work
pMD04	pBAD18 carrying ami2_Di, Kan^R	This work
pMD05	pET28b carrying ami1_Di, Kan^R	This work
pMD06	pET28b carrying ami2_Di, Kan^R	This work
Strain
D. indicus
DR1	Wild-type	[35]
E. coli
DH5α	Φ80 ΔlacZΔM15 Δ(lacZYA-argF)U169 deoR recA1 endA hsdR17(rk-,mk+) phoA supE44 thi-1 gyrA96 relA1	Laboratory strain collection
BL21 (DE3)	F⁻ompT hsdS_B(r_B^–, m_B⁻) gal dcm (DE3) pLysS(Cam^R)	Laboratory strain collection
MG1655	K-12 F⁻λ⁻ilvG⁻rfb-50 rph-1	Laboratory strain collection
RP21	MG1655 ΔamiA::frt ΔamiC::frt /pBAD18	[30]
RP101	MG1655 ΔamiA::frt ΔamiC::frt /pMD01	This work
RP102	MG1655 ΔamiA::frt ΔamiC::frt /pMD02	This work
RP103	MG1655 ΔamiA::frt ΔamiC::frt /pMD03	This work
RP104	MG1655 ΔamiA::frt ΔamiC::frt /pMD04	This work
RP105	BL21(DE3)/pMD05	This work
RP106	BL21(DE3)/pMD06	This work

ISSN: 1471-2180