Fig. 6

Peptidoglycan hydrolytic cleavage activity of Ami1_Di and Ami2_Di. (A) Western blot showing purified His-tagged Ami2_Di (65.6 kDa) – Lane 1 and Ami1_Di (42.5 kDa) – Lane 2 protein heterologously produced in E.coli BL21(DE3) strain (RP106 & RP105). M- Protein marker. (B) Determination of the PG hydrolase activity of Ami1_Di and Ami2_Di. Remazol brilliant blue (RBB)-labeled peptidoglycan was incubated with Ami1_Di (2 µM and 4µM) and Ami2_Di (2 µM and 4 µM), lysozyme as positive control and buffer as negative control at 37 °C. At 1 h and 12 h, the samples were pelleted down, and the absorbance of the supernatant was measured at 595 nm. The pictures below the panel show the results with an incubation of 12 h. Three biological replicate experiments were performed for each reaction, with error bars representing the standard deviation