Fig. 4

Overexpression of ami2_Di leads to cell lysis in E. coli. (A) Phase contrast images of strain RP101 and RP104 showing lytic activity of ami1_Di and ami2_Di, respectively on overexpressing under the control of L-arabinose promoter. Strain RP21, RP101 and RP104 were grown till OD₆₀₀ ∼ 0.2 and induced with L-arabinose for 10 h. Cells were collected, and about 6 µL sample was immobilized on 1XPBS agarose pad and imaged. Cells overexpressing ami2_Di (RP104) show higher lytic activity as represented by dead cells. (B) Growth curve assay confirming the elevated lytic activity of ami2_Di (RP104). Strain RP21, RP101, and RP104 were induced with L-arabinose at OD₆₀₀ ∼ 0.1. Optical density was measured every 1 h. Cells induced with 0.2% glucose were used as the negative control. Datasets are from three biological replicates, and error bars represent standard deviation