Fig. 3
D. indicus Ami2Di restores cytokinesis in E. coli amidase mutant. (A) Phase contrast micrographs representing cells of strain RP21 (MG1655 ΔamiA::frt ΔamiC::frt /pBAD18), RP104 (MG1655 ΔamiA::frt ΔamiC::frt /pBAD18ami2Di) induced with 0.4% L-arabinose. The complementation of E. coli amidase mutant by ami2Di with 0.4% L-arabinose reduced chaining. (B) Quantitative analysis of phase contrast micrographs of strains RP21 and RP104. On induction with L-arabinose, RP104 cells show a significant reduction in chains and increment in singlets and doublets cells. Cells were counted as mentioned in Materials and Methods. Datasets are from three independent experiments, and error bars represent standard deviation. P value = RP21 vs. RP104 + 0.2% glu (glucose), p > 0.05*, RP21 vs. RP104 + 0.4% ara (arabinose), p < 0.05**. No. of cells – Total number of cells counted for each strain