Table 1 The HCV-RT-LAMP-AuNPs-LFB primers used in this study
Primer name | Sequence and modifications | Length | Gene |
|---|---|---|---|
F3 | 5’-TCGT(A/G)CAGCCTCCAGG(A/C)-3’ | 17 nt | 5’UTR |
B3 FIP | 5’-GGTCTACGAGACCTCCCG-3’ 5’-AAGAAAGGACCC(A/G)GTC(G/A/T)(C/T)CC(C/T)-CATAGT(A/G)GTCTGCGGAACC-3’ | 18 nt 40 mer | |
FIP* | 5’-FAM-AAGAAAGGACCC(A/G)GTC(G/A/T)(C/T)CC(C/T)-CATAGT(A/G)GTCTGCGGAACC-3’ | 40 mer | |
BIP LF LF* LB | 5’-CCGC(A/G)AGA(C/T)(C/T)(A/G)CTAGCCGA-GCACCCTATCAGGCAGTACC-3’ 5’-C(A/G)ATTCCGGTGTACTCAC-3’ 5’-Biotin-C(A/G)ATTCCGGTGTACTCAC-3’ 5’-TAG(C/T)GTTGGGT(C/T)GCGAAAG-3’ | 39 mer 18 nt 18 nt 19 nt |