Fig. 6

HCV-RT-LAMP-AuNPs-LFB assay specificity with different strains. Assay specificity was evaluated using different nucleic acid templates. Each result was tested through AuNPs-LFB: 1–5, HCV 1b, 2a, 3b, 6a, and 3a 5’UTR plasmids; 6–11, HCV clinical samples; 12, Hepatitis B virus; 13, Human immunodeficiency virus; 14, Epstein-Barr virus; 15, Coxsackie virus CAV16; 16, Human papillomavirus; 17, Human enterovirus EV71; 18, Influenza A virus; 19, Influenza B virus; 20, Cryptococcus neoformans; 21, Enterococcus faecium; 22, Salmonella enteritidis; 23, Shigella bogdii; 24, Staphylococcus aureus; 25, Streptococcus pyogenes; 26, Streptococcus pneumoniae; 27, Escherichia coli; 28, Pseudomonas aeruginosa; 29, Klebsiella pneumoniae; 30, Mycobacterium tuberculosis; 31, Brucella; 32, Other microbial nucleic acid mixtures containing HCV-1b plasmids; 33, Other microbial nucleic acid mixtures containing HCV-2a plasmids; 34, Other microbial nucleic acid mixtures containing HCV-3b plasmids; 35, Other microbial nucleic acid mixtures containing HCV-6a plasmids; 36, Other microbial nucleic acid mixtures containing HCV-3a plasmids; 37, Other microbial nucleic acid mixtures; 38, distilled water (DW). CL, control line; TL, test line