Fig. 4
Sensitivity analysis of the HCV-RT-LAMP-AuNPs-LFB assay with serial dilutions of nucleic acid template. Visual reagent (L-HNB) and AuNPs-LFB approaches were simultaneously used to readout the HCV-RT-LAMP outcomes. L-HNB (A)/AuNPs-LFB (B); 1–6 represent the HCV plasmid concentrations of 2.0 × 103 copies, 2.0 × 102 copies, 2.0 × 101 copies, 2.0 × 100 copies, and 1 copy per test and distilled water (DW), respectively. The limit of detection (LoD) of the HCV-RT-LAMP assay was 20 copies per test. CL: control line; TL: test line