Fig. 2

(A) Confocal microscopy showing that Lac-EVs were engulfed by MG. The MG cells were coincubated with PKH26-labeled Lac-EVs for 12 h at room temperature. Lac-EVs were labeled with PKH26, and the other material was labeled with DAPI. (B) Cell morphology at different concentrations. (C) Cell viability of different groups under different concentrations and time points. (D) q-PCR analysis of IL-6, IL-10, and IL-1β at a concentration of 2.5 µg/mL and after culture for 24 h. The data are expressed as the mean fold change ± SEM of triplicate measurements, and statistical significance was analyzed by one-way ANOVA: *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001