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Table 4 Comparison of different PCR annealing temperatures, primer sets, and Taq polymerases, and their influence on DNA amplification

From: Optimized bacterial community characterization through full-length 16S rRNA gene sequencing utilizing MinION nanopore technology

 

Polymerases

LongAmp

iTaq

Primer set

Annealing Temperature

(°C)

Read length

(bp)

Sequence

Number (× 104)

Read length

(bp)

Sequence

Number (× 104)

Set#1

48

1′457

2.35

1′451

2.10

50

1′458

2.99

1′450

2.69

52

1′455

2.20

1′447

2.05

Average ± SD

1′457 ± 1.5

2.51 ± 0.42

1′449 ± 2.1

2.28 ± 0.36

Set#2

48

1′458

2.92

1′451

2.15

50

1′454

3.68

1′448

2.86

52

1′455

3.45

1′448

1.40

Average ± SD

1′456 ± 2.1

3.35 ± 0.39

1′449 ± 1.7

2.14 ± 0.73

  1. Equal amounts of DNA were sequenced for each sample
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BMC Microbiology

ISSN: 1471-2180

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