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Table 2 Number of genera identified per workflow and number of PCR cycles. No minimal thresholds were set

From: Optimized bacterial community characterization through full-length 16S rRNA gene sequencing utilizing MinION nanopore technology

16S Databases

Silva

RefSeq

Workflows

Kraken2

EPI2ME

BugSeq

16S

WIMP

PCR cycles

15

71

197

252

8

20

74

206

265

8

25

89

211

291

8

30

76

214

286

8

35

84

209

261

9

  1. The Epi2me and 16S workflow had a minimum identification accuracy of 80%. PCR was conducted using an annealing temperature of 48°C, primer set#1, and LongAmp Taq polymerase
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BMC Microbiology

ISSN: 1471-2180

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