Table 1 Percentage of misclassified reads per workflow and the number of PCR cycles. Misclassified reads included all false positives, unclassified reads, and those classified higher than the genus classification
16S Databases | Silva | RefSeq | |||
|---|---|---|---|---|---|
Workflows | Kraken2 | EPI2ME | BugSeq | ||
16S | WIMP | ||||
PCR cycles | 15 | 2.6% | 2.3% | 14.3% | 0.8% |
20 | 2.7% | 2.2% | 14.6% | 1.3% | |
25 | 2.8% | 2.4% | 14.7% | 1.5% | |
30 | 3.4% | 2.7% | 14.4% | 3.5% | |
35 | 4.4% | 3.5% | 14.3% | 10.8% | |