Fig. 2

A representative agarose gel (0.8%) showing amplified 16S gene DNA (~ 1,500 bp) after 15-20-25-30-35 PCR cycles. PCR amplification was performed using the LongAmp polymerase and primer set#1. (A, DNA concentrations are shown below). In the bar graph, four workflows, namely Kraken2-Silva, EPI2ME-16S, EPI2ME-WIMP, and BugSeq, were compared to determine the relative abundance of microbial genera in the mock community based on increasing PCR cycles (B). (MC = microbial community, others = misclassified sequences)