Fig. 6

Assay evaluation using A. fumigatus spiked clinical respiratory samples. A sputum sample (previously negative for Aspergillus by ITS and TEF sequencing) spiked with varying quantities (2, 10 and 25 haploid genome equivalents/µl) of A. fumigatus gDNA. (A) Fungal read counts are shown for each sample in quadruplicate. Samples with less than 500 fungal reads were removed. No template control and unspiked sputum sample did not produce significant fungal read yields. (B) Mean A. fumigatus counts displayed a linear relationship with amount of spiked A. fumigatus (error bars indicated standard error). R2 value for linear relationship is indicated