Fig. 1

In silico analyses of ITS1 and TEF1α targets. (A) PrimerTree was used to search primers against fungal sequences within the NCBI nr nucleotide database. The frequency of product lengths are displayed for each target (vertical dotted line indicates median). (B) A curated database of TEF sequences was established. The 8656 sequences cover the five phyla containing known human pathogenic fungi and the number of species covered within these phyla are shown. (C) ITS1 (left) and TEF1α sequences were obtained for over thirty clinically relevant fungal species and alignments performed using Clustal to demonstrate fungal speciation. Phylogenetic trees were generated using maximum-likelihood method and Tamura-Nei model