Fig. 4

Analysis of the SCCmec subtype of MRSA isolated from clinical samples of patients with diabetic foot infections. A Antimicrobial sensitivity testing was conducted on 32 clinical samples that were tested positive for both femA and mecA in the LAMP detection. B Analysis of 14 S. aureus strains isolated from clinical samples was conducted using the LAMP method targeting the mecA gene. These clinical samples were found to be positive for mecA and negative for drug sensitivity testing. C Schematic diagram of LAMP primers for SCCmec type II. The target of this primer is located at the 5' end of the orfx gene of S. aureus and at the 3' end of the SCCmec element. FIP (forward inner primer): F1c + F2, BIP (backward inner primer): B1c + B2. F1c: F1 complementary strand, B1c: B1 complementary strand. D Analysis of 32 clinical samples that were tested positive for both femA and mecA using LAMP primers targeting type II and type III SCCmec components. E Comparison of the gene sequences of two other subtypes of MRSA strains with type II SCCmec. The gene sequences of these two strains were obtained through high-throughput sequencing