Table 1 Purification of ADI from P. chrysogenum
From: Purification and characterization of L-arginine deiminase from Penicillium chrysogenum
Purification step | Total protein (mg) | Total activity (U) | Specific activity (Umg− 1) | Yield (%) | Fold of purification |
|---|---|---|---|---|---|
Crude extract | 300 | 240 | 0.8 | 100 | 1.0 |
85% (NH4) 2 SO4 | 55 | 35 | 0.6 | 14.6 | 0.8 |
DEAE-Cellulose | 16 | 22 | 1.4 | 9.2 | 1.7 |
Sephadex G-200 | 0.3 | 15 | 50.0 | 6.3 | 62.5 |