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Fig. 5 | BMC Microbiology

Fig. 5

From: Development of a multiple cross displacement amplification combined with nanoparticles-based biosensor assay for rapid and sensitive detection of Streptococcus pyogenes

Fig. 5

Optimal detection time for the S. pyogenes MCDA-LFB assay. Four reaction times (A, 10 min; B, 20 min; C, 30 min; D, 40 min) were selected to determine the optimal detection time at 63 °C. Tubes/biosensors 1- 8 represent S. pyogenes strain ATCC19615 DNA levels of 2 ng, 200 pg, 20 pg, 2 pg, 200 fg, 20 fg and 2 fg per reaction, and a blank control (DW), respectively. The minimum detection limit was observed when MCDA lasted for 30 min (C)

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BMC Microbiology

ISSN: 1471-2180

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