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Fig. 4 | BMC Microbiology

Fig. 4

From: Development of a multiple cross displacement amplification combined with nanoparticles-based biosensor assay for rapid and sensitive detection of Streptococcus pyogenes

Fig. 4

Sensitivity of the LFB assay for S. pyogenes-MCDA products Measurement techniques of colorimetric indicator (A), gel electrophoresis (B) and lateral flow biosensor (C) were used to analyze MCDA amplicons. A tenfold serial dilutions of the template in a range between 2 ng and 2 fg were prepared for evaluating the detection line.Tubes (A)/Lanes (B)/Biosensors (C) 1–8 respectively represent S. pyogenes strain ATCC19615 DNA levels of 2 ng, 200 pg, 20 pg, 2 pg, 200 fg, 20 fg and 2 fg per reaction, and a blank control (DW)

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