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Fig. 3 | BMC Microbiology

Fig. 3

From: Development of a multiple cross displacement amplification combined with nanoparticles-based biosensor assay for rapid and sensitive detection of Streptococcus pyogenes

Fig. 3

Optimization of amplification temperature for S. pyogenes-MCDA primers 20 pg of S. pyogenes genome added to the MCDA reaction was incubated in a range between 60 °C and 66 °C for 30 min. Two methods, including MG and agarose gel electrophoresis, were then used to evaluate the amplification efficiency of the S. pyogenes-MCDA assays. Tubes/agarose gel electrophoresis 1–7 represent 60 °C, 61 °C, 62 °C, 63 °C, 64 °C, 65 °C, 66 °C respectively

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BMC Microbiology

ISSN: 1471-2180

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