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Fig. 3 | BMC Microbiology

Fig. 3

From: Evaluation of a novel lysis-based sample processing method to optimize Vibrio vulnificus detecting by loop-mediated isothermal amplification assay

Fig. 3

LAMP results on artificially contaminated oyster samples. (A), (B) and (C) the DNA templates were prepared via the water-lysis, spin filtration and boiling methods respectively, the representative LAMP results showed that V. vulnificus 1H00066 presented “S” curves at 103~109 CFU/g. N.C, negative control; P.C, positive control. (D), (E) and (F) the DNA templates were prepared via the water-lysis, spin filtration and boiling methods respectively, the representative PCR results showed 103~109 CFU/g. A band of 233 bp was seen with positive samples. Lane 1 is 100 bp molecular weight marker; Lanes 2–11, (109 CFU/g, 108 CFU/g, 107 CFU/g, 106 CFU/g, 105 CFU/g, 104 CFU/g, 103 CFU/g, 102 CFU/g, 101 CFU/g and 100 CFU/g, respectively). The negative control used was water (not shown). In gels, 5 µL of PCR amplicons were loaded per lane

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BMC Microbiology

ISSN: 1471-2180

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