Fig. 1
LAMP and PCR sensitivity results via three sample processing methods. (A), (B) and (C) the DNA templates were prepared via the water-lysis, spin filtration and boiling methods respectively, the representative LAMP results showed that V. vulnificus 1H00066 presented “S” curves at 102~109 CFU/mL. N.C, negative control; P.C, positive control. (D), (E) and (F) the DNA templates were prepared via the water-lysis, spin filtration and boiling methods respectively, the representative PCR results showed that V. vulnificus 1H00066 presented at 103~109 CFU/mL. A band of 233 bp was seen with positive samples. Lane 1 is 100 bp molecular weight marker; Lanes 2 ~ 11, amplification products using 10-fold serial dilutions of a bacterial culture (109 CFU/mL, 108 CFU/mL, 107 CFU/mL, 106 CFU/mL, 105 CFU/mL, 104 CFU/mL, 103 CFU/mL, 102 CFU/mL, 101 CFU/mL and 100 CFU/mL, respectively). The negative control used was water (not shown). In gels, 5 µL of PCR amplicons were loaded per lane