Table 1 Primers used in this study
Gene | Gene product | Oligonucleotide sequence | Product size (bp) | Annealing | Reference |
|---|---|---|---|---|---|
adk | adenylate kinase | CATCATTCTTCTCGGTGCTC | 592 | 59℃ | [8] |
AGTGCCGTCAAACTTCAGGTA | |||||
gyrB | DNA gyrase subunit B | GTACGTTTCTGGCCTAGTGC | 749 | 60℃ | [8] |
GGGTCTTTTTCCTGACAATC | |||||
metE | methionine synthase | CGGGTGACTTTGCTTGGT | 827 | 58℃ | [8] |
CAGATCGACTGGGCTGTG | |||||
mdh | malate dehydrogenase | ATGAAAGTCGCTGTTATTGG | 692 | 60℃ | [8] |
GCCGCTTGGCCCATAGAAAG | |||||
pntA | pyridine nucletide transhydrogenase | GGCCAGCCCAAAATCCT | 758 | 59℃ | [8] |
GATATTGCCGTCTTTTTCTT | |||||
purM | phosphoribosylformylglycinamide cyclo-ligase | GGTGTCGATATTGATGCAGG | 734 | 58.4℃ | [8] |
GGAATGTTTTCCCAGAAGCC | |||||
pyrC | Dihydroorotase | ATCATGCCTAACACGGTTCC | 726 | 56℃ | [8] |
TTCAAACACTTCGGCATA | |||||
ctxA/B | Cholera toxin | TATGCCAAGAGGACAGAGTGAG | 115 | 55℃ | [27] |
AACATATCCATCATCGTGCCTAAC | |||||
tcpA | Toxin co-regulated pilus subunit A | GGTCAGCCTTGGTAAGGTT | 225 | 50℃ | This study |
CAAGATCAGCGACAGCAG |