Table 1 PCR primer sequences used for the molecular identification of L. monocytogenes isolates and detection of their virulence genes
Target gene | Primer name | Oligonucleotide sequences | Amplicon (bp) | Reference |
|---|---|---|---|---|
16S rRNA | 16S rRNA (F) 16S rRNA (R) | 5′- CAGCAGCCGCGGTAATWC-3′ 5′- CTCCATAAAGGTGACCCT − 3’ | 938 | [23] |
iap | iap (F) | 5′ ACAAGCTGCACCTGTTGCAG ′3 | 131 | [24] |
iap (R) | 5′ TGACAGCGTGTGTAGTAGCA ′3 | |||
hylA | hlyA (F) | 5′ GCAGTTGCAAGCGCTTGGAGTGAA ′3 | 456 | [24] |
hlyA (R) | 5′ GCAACGTATCCTCCAGAGTGATCG ′3 | |||
actA | actA (F) | 5′ CGCCGCGGAAATTAAAAAAAGA ′3 | 839 | [24] |
actA (R) | 5′ ACGAAGGAACCGGGCTGCTAG ′3 |