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Table 5 Modified Knott’s test versus cPCR and Sanger sequencing agreement statistics for the two most common filarial worm pathogens identified from 100 Sri Lanka dog blood samples

From: Development and validation of a long-read metabarcoding platform for the detection of filarial worm pathogens of animals and humans

Filarial worm

Modified Knott’s test

cPCR and Sanger sequencing

Total agreement (%)

Kappa (95% CI)

Agreement

Kappa SE

NEG

POS

Brugia spp.

NEG

92

6

94

0.38

(0.187–0.573)

Fair

< 0.001

POS

0

2

Dirofilaria spp.

NEG

66

15

83

0.562

(0.472–0.652)

Moderate

< 0.001

POS

2

17

  1. POS = positive, NEG = negative, CI = confidence intervals, SE = standard error. Agreement level defined as poor if coefficient (k) is ≤ 0.20, fair agreement if 0.21 ≤ k ≤ 0.40, moderate agreement if 0.41 ≤ k ≤ 0.60, substantial agreement if 0.61 ≤ k ≤ 0.80, and high agreement if k > 0.81. Genus-level taxonomic classifications are shown as the modified Knott’s test can only identify microfilaria to this taxonomic level, whilst cPCR and Sanger sequencing could classify down to species level in all instances
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BMC Microbiology

ISSN: 1471-2180

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