Table 1 Performance of our COI gene-targeting metabarcoding assay and NanoCLUST pipeline classification on reference filarial worm controls
Filarial worm pathogen(s) present | Sample type | NanoCLUST classification | NCBI accession no. | Length (bp) | Identity (%) | No. of reads |
|---|---|---|---|---|---|---|
Acanthocheilonema reconditum | Canine blood | Acanthocheilonema reconditum | JF461456.1 | 496 | 99.6 | 22,241 |
Breinlia boltoni | Whole adult worm from possum lung | Breinlia boltoni | OP040125.1 | 650 | 100 | 46,652 |
Brugia sp. Sri Lanka (SL) genotype | Canine blood | Brugia sp. Sri Lanka (SL) genotype | MN564741.1 | 630 | 99.7 | 99,070 |
Brugia sp. SL genotype and Dirofilaria sp. ‘hongkongensis’ | Canine blood | Brugia sp. SL genotype, Dirofilaria sp. ‘hongkongensis’ | MN564741.1, KX265050.1 | 664, 679 | 99.7, 99.9 | 2172; 76,144 |
Cercopithifilaria rugosicauda | Tick haemolymph | Cercopithifilaria rugosicauda | KC610815.1 | 514 | 99.8 | 88,207 |
Dipetalonema gracile | Whole worm from monkey abdominal cavity | Dipetalonema gracile | KP760179.1 | 628 | 98.7 | 75,145 |
Dirofilaria immitis | Canine blood | Dirofilaria immitis | MW577348.1 | 688 | 99.7 | 5695 |
Dirofilaria repens and Dirofilaria immitis | Canine blood | Dirofilaria repens, Dirofilaria immitis | MW675691.1, MW577348.1 | 668, 687 | 99.9, 99.6 | 7923; 69,844 |
Dirofilaria sp. ‘hongkongensis’ | Canine blood | Dirofilaria sp. ‘hongkongensis’ | KX265050.1 | 600 | 99.8 | 89,455 |
Onchocerca gibsoni and Stephanofilaria sp. | Lesion swab from cow | Onchocerca gibsoni, Stephanofilaria sp. | AJ271616.1, MW143322.1 | 649, 425 | 99, 100 | 218; 675 |
Onchocerca lupi | Clinical sample, whole adult worm from canine eye | Onchocerca lupi | JX080029.1 | 686 | 100 | 72,533 |
Setaria tundra | Mosquito haemolymph | Setaria tundra | KF692103.1 | 676 | 99.9 | 66,504 |
Wuchereria bancrofti | Whatman FTA card blood spot from human | Wuchereria bancrofti | AP017705.1 | 684 | 99.4 | 78,220 |