No. | Medium components |
---|
1. | S-Screening medium 1 |
2. | L-LB, S-LB 2 |
3. | L-MRS, S-MRS 3 |
4. | TGFG L-MRS4 |
5. | GFFG L-MRS 5 |
6. | HGFG L-MRS 6 |
- 1 S-Screening medium: the solid media with 0.5% (w/v) (NH4)2SO4, 0.1% (w/v) KH2PO4, 0.1% (w/v) NaCl, 0.05% (w/v) MgSO4.7H2O, 0.01% (w/v) CaCl2, 0.02% (w/v) yeast extract, 1.5% (w/v) agar and 0.1% (w/v) free gossypol as the sole carbon source;
- 2 L-LB: the liquid media prepared from the composition of standard LB broth (China Haibo Biotechnology Co., Ltd., Qingdao, China). S-LB: the solid media with L-LB medium and 1.5% (w/v) agar;
- 3 L-MRS: the liquid media prepared from the composition of standard Man-Rogosa-Sharpe (MRS) broth (China Haibo Biotechnology Co., Ltd., Qingdao, China). S-MRS: the solid media with L-MRS medium and 1.5% (w/v) agar;
- 4 TGFG L-MRS: the L-MRS medium with 0.01% (w/v) free gossypol;
- 5 GFFG L-MRS: the L-MRS medium with glucose-free but 0.01% (w/v) free gossypol;
- 6 HGFG L-MRS: the L-MRS medium with half glucose (1% (w/v) glucose) and 0.01% (w/v) free gossypol;