Fig. 4

ARG detection in low complexity bacterial metagenomes. Synthetic metagenomes (n = 50) consisting of short-reads from five organisms mixed at different relative proportions (0.1-, 1-, 2-, 5-, and 10-X genome coverage; n = 10 at each coverage level) were evaluated for presence of ARGs using KMA (□), CARD-RGI (Ο), and SRST2 (✕) in silico tools. Percent ARG detection (y-axis) in 10 replicates as a function of target gene template coverage (x-axis) is shown. Point color differentiates between organism and ARG-detection tool used (see legend). Where multiple points of the same colour/shape are present for a given template-identity range (x-axis), each point represents a different allele. Detection greater than 100% indicates detection of multiple alleles, rather than only the target allele