Fig. 1

Incorrect assignment of operational taxonomic units (OTUs) to closely related genera. A Assigned OTUs (y-axis) as a function of target isolate’s genome coverage (x-axis). Analyses were conducted on subsampled reads of each target-genus (top-panel headings) and grouped by genus (color legend). For each coverage level (0.1, 1, 2, 5, or 10X) n = 10 subsampled replicates of the target organism were created. Lines represent the linear regression (log (y + 0.1) ~ log(x)) fit to each genus (see legend). B to F: Pairwise comparisons between top 10 genera with mapped OTUs and subsampled targets: B. Enterococcus, C. Escherichia, D. Klebsiella, E. Listeria, and F. Salmonella. Points represent the modelled slope of the regression analysis ± 95% confidence intervals (y-axis). Target organism is indicated by a red circle and red text (x-axis). Significance values are displayed above select data points of interest: p > 0.05 = ns; p < 0.05 = *; p < 0.01 = **; p < 0.001 = ***