Fig. 8
From: Rapid intestinal and systemic metabolic reprogramming in an immunosuppressed environment
Changes in the distribution of lymphocytes and myeloid cells and LN structure due to abx and tacrolimus. Mice given abx (6 days) with or without 2 days of tacrolimus. Representative qualitative heatmaps of IHC marker changes relative to untreated control (red = increased; blue = decreased; white = unchanged) (a) mLN, (b) pLN, and (c) intestine. Graphs of individual IHC values for each marker and tissue type listed in Supplementary Fig. 12. (d) In vivo gut permeability assay performed using FITC-Dextran. Mice were gavaged with FITC-Dextran and serum samples were collected post 4 h of administration. 3 mice/ group, at least 2 mLN, pLN, and sections of intestine at duodenal-jejunal junction/mouse, 3 sections/staining panel. Ordinary one-way ANOVA with Tukey’s multiple comparisons test. Representative of 2 repeated experiments. * p < 0.05; ** p < 0.01, *** p < 0.001, **** p < 0.0001. Abbreviations: LN: lymph node; mLN: mesenteric LN; pLN: peripheral LN; IHC: immunohistochemistry