The effects of Leishmania RNA virus 2 (LRV2) on the virulence factors of L. major and pro-inflammatory biomarkers: an in vitro study on human monocyte cell line (THP-1)

Table 1 Primer sequences for parasite VF genes and pro-inflammatory biomarkers

Genes	Primer name	Sequence 5’-3’	Length (mer)	Tm	CG (%)	Amplification size(bp)	Refs
Alpha tubulin	ALT-F	CAGGTGGTTGTCGTCTCTGAC	20	60.04	60	119	(17)
Alpha tubulin	ALT-R	TAGCTCGTCAGCACGAAGTG	20	60.11	55	119
GP63	GP63-F	ATCTGTGGCGACTTCAAGGT	20	59.31	50	136
GP63	GP63-R	CAGAGAACGTCTGGCAGGTC	20	60.39	60	136
MPI	MPI-F	AGTGCCCTACCTGCTGAAGA	20	59.87	55	138
MPI	MPI-R	ATGAGCTCTGGCTTGTGGTT	20	59.31	50	138
HSP-83	HSP83-F	ACGAAGCACTTCTCTGTGGAG	21	59.66	53.38	108
HSP-83	HSP83-R	GATGTTGTTGCGCTTCTTGTT	21	60.31	42.38	108
Β-actin	B-act-F	ATGTGGCCGAGGACTTTGATT	21	60.00	47.62	107	(38)
Β-actin	B-act-R	AGTGGCCGAGGACTTTGATT	21	59.99	52.38	107
NLRP3	NLRP3-F	AAGGAAGTGGACTGCGAGA	19	58.25	52.63	127
NLRP3	NLRP3-R	TCAAACGACTCCCTGGAAC	19	57.00	52.63	127
IL18	IL18-F	ATCGGCCTCTATTTGAAGATATGACT	26	59.79	38.46	100
IL18	IL18-R	CCTCTAGGCTGGCTATCTTTATACATACT	29	61.46	41.38	100
IL1β	IL1β-F	CAGGGACAGGATATGGAGCAAC	22	60.49	54.55	133
IL1β	IL1β-R	CATCTTTCAACACGCAGGACAG	22	60.10	50	133

ISSN: 1471-2180