Fig. 3From: Targeted elimination of Vancomycin resistance gene vanA by CRISPR-Cas9 systemRelative copy number of the plasmid pUC19-vanA (20 and 30 bp) at each time point. pCas9-sgRNA (20 and 30 bp) and pCas9 were transformed into competent E.coli C600 + pUC19-vanA as the experimental group and the control group, respectively. The sgRNA (20 and 30 bp) are the lengths of the sgRNA targeting the vanA resistance geneBack to article page