Fig. 2
From: Targeted elimination of Vancomycin resistance gene vanA by CRISPR-Cas9 system
PCR amplification using primers vanA-F/R to identify pCas9-sgRNA elimination of vanA gene in E. coli DH5α + pUC19-vanA. (A) The E.coli DH5α + pUC19-vanA strain transformed with pCas9-sgRNA (B) and the E.coli DH5α + pUC19-vanA strain transformed with pCas9. Markers with an M value of 2,000 bp. P represents the E. coli DH5α + pUC19-vanA strain in which pCas9 is a positive control. N indicates a negative control. Markers with an M value of 2,000 bp. P represents the E. coli DH5α + pUC19-vanA strain in which pCas9 is a positive control and N denotes negative control