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Table 3 Transcription rates of genes involved in basic metabolisma

From: Genome-wide transcriptional response to silver stress in extremely halophilic archaeon Haloferax alexandrinus DSM 27206 T

Hfx. alexandrinus DSM 27206 ORFb

Gene name

Log2FC

Annotation

0.1 mM

0.25 mM

0.5 mM

Carbohydrate metabolim

Hfx-2019-SC1-cds280

 

-

-

2.23

ABC.SS.P simple sugar transport system permease protein

Hfx-2019-SC1-cds281

 

-

-

2.22

ABC.SS.P simple sugar transport system permease protein

Hfx-2019-SC1-cds378

gatC

2.51

-

-

Galactitol-specific IIC component

Hfx-2019-SC2-cds179

ppsA

2.13

-

-

Pyruvate water dikinase

Hfx-2019-SC2-cds823

 

-

-

3.49

COG0701 Predicted permeases

Hfx-2019-SC2-cds834

fbp

3.74

-

-

Fructose-1 6-bisphosphatase I

Hfx-2019-SC3-cds294

gap2

2.53

-

-

Glyceraldehyde-3-phosphate dehydrogenase (NAD(P))

Hfx-2019-SC5-cds125

galE

3.83

-

3.63

UDP-glucose 4-epimerase

Hfx-2019-SC5-cds135

ich-Y

-

-

2.2

Itaconyl-CoA hydratase

Hfx-2019-SC1-cds496

ugpB

-

-

-2.01

sn-glycerol 3-phosphate transport system substrate-binding protein

Hfx-2019-SC1-cds980

iolG

-2.32

-

-

Myo-inositol 2-dehydrogenase / D-chiro-inositol 1-dehydrogenase

Hfx-2019-SC2-cds552

 

-

-2.27

-

AAA-type ATPase, core component

Hfx-2019-SC2-cds877

gnaD

-2.9

-

-

Gluconate/galactonate dehydratase

Hfx-2019-SC3-cds298

gapA

-2.47

-

-

Glyceraldehyde 3-phosphate dehydrogenase

Hfx-2019-SC3-cds336

gtsA

-3.46

-

-

Glucose/mannose transport system substrate-binding protein

Hfx-2019-SC3-cds337

gtsB

-3.83

-

-

Glucose/mannose transport system permease protein

Hfx-2019-SC3-cds338

gtsC

-3.06

-

-

Glucose/mannose transport system permease protein

Hfx-2019-SC3-cds339

msmX

-3.78

-

-

Multiple sugar transport system ATP-binding protein

Hfx-2019-SC3-cds350

araE

-

-

-2.44

Arabinose:H+ symporter

Hfx-2019-SC4-cds36

iolE

-

-

-6.43

Sugar phosphate isomerases/epimerases

Hfx-2019-SC4-cds78

msmX

-

-2.34

-

Multiple sugar transport system ATP-binding protein

Hfx-2019-SC4-cds146

thuA

-

-2.92

-4.28

Trehalose utilization protein

Hfx-2019-SC4-cds211

gtsC

-

-

-5.63

Glucose/mannose transport system permease protein

Lipid metabolim

Hfx-2019-SC2-cds258

acs

2.2

-

2.5

AMP-forming acetyl-CoA synthetase

Hfx-2019-SC2-cds631

acs

2.39

-

-

AMP-forming acetyl-CoA synthetase

Hfx-2019-SC2-cds779

caiC

2.01

-

-

Fatty-acyl-CoA synthase

Hfx-2019-SC5-cds129

 

2.6

-

-

Enoyl-CoA hydratase

Hfx-2019-SC5-cds130

paaH

2.82

-

4.02

3-hydroxybutyryl-CoA dehydrogenase

Hfx-2019-SC5-cds131

pksG

3.19

-

3.59

hydroxymethylglutaryl-CoA synthase

Hfx-2019-SC5-cds132

paaJ

2.28

-

3.15

Acetyl-CoA C-acetyltransferase

Hfx-2019-SC5-cds133

paaK

3.3

-

3.49

Phenylacetate-CoA ligase

Hfx-2019-SC5-cds134

paaI

2.86

-

3.83

Acyl-CoA thioesterase

Hfx-2019-SC5-cds136

epi

2.14

-

2.16

Methylmalonyl-CoA/ethylmalonyl-CoA epimerase

Hfx-2019-SC5-cds142

paaI

2.75

-

2.95

Acyl-CoA thioesterase

Hfx-2019-SC5-cds145

paaD

2.9

-

-

Predicted metal-sulfur cluster biosynthetic enzyme

Hfx-2019-SC5-cds146

paaC

3.17

-

2.45

Ring-1,2-phenylacetyl-CoA epoxidase subunitPaaC

Hfx-2019-SC5-cds147

paaB

3.17

-

2.44

Ring-1 2-phenylacetyl-CoA epoxidase subunitPaaB

Hfx-2019-SC5-cds148

paaA

2.69

-

-

Ring-1 2-phenylacetyl-CoA epoxidase subunit PaaA

Hfx-2019-SC5-cds76

prpD

-

-4.03

-

2-methylcitrate dehydratase

Hfx-2019-SC5-cds77

prpD

-

-3.36

-

2-methylcitrate dehydratase

Amino acid metabolism

Hfx-2019-SC1-cds368

puuE

2.81

-

2.17

4-aminobutyrate aminotransferase

Hfx-2019-SC2-cds36

pdhC

2.56

-

-

Pyruvate dehydrogenase E2 component

Hfx-2019-SC4-cds252

hyuA

-

-

2.19

N-methylhydantoinases

Hfx-2019-SC5-cds98

hutH

2.17

-

-

Histidine ammonia-lyase

Hfx-2019-SC5-cds118

hyuA

-

-

2.30

N-methylhydantoinase

Hfx-2019-SC5-cds119

hyuB

2.18

-

2.23

N-methylhydantoinases

Hfx-2019-SC5-cds120

pepQ

3.56

-

2.11

Xaa-Pro dipeptidase

Hfx-2019-SC5-cds126

ilvB

3.59

-

4.02

Acetolactate synthase I/II/III large subunit

Hfx-2019-SC5-cds151

 

2.1

-

-

Amidohydrolase

Hfx-2019-SC1-cds54

RP-S6e

-2.72

-

-

RPS6 small subunit ribosomal protein S6e

Hfx-2019-SC3-cds359

livK

-

-

-2.59

Branched-chain amino acid transport system substrate-binding protein

Hfx-2019-SC4-cds106

ilvA

-

-2.53

-2.05

Threonine dehydratase

Hfx-2019-SC4-cds221

 

-

-2.08

-

Aspartate aminotranferase family member

Hfx-2019-SC4-cds222

dat

-

-2.99

-2.48

Diaminobutyrate-2-oxoglutarate transaminase

Hfx-2019-SC5-cds223

pydC

-

-

-2.05

Beta-ureidopropionase/ N-carbamoyl-L-amino-acid hydrolase

Hfx-2019-SC6-cds119

lysK

-2.16

-

-2.15

LysW-gamma-L-lysine/LysW-L-ornithine carboxypeptidase

Hfx-2019-SC6-cds120

lysJ

-2.01

-

-

LysW-gamma-L-lysine/LysW-L-ornithine aminotransferase

Nucleotide metabolism

Hfx-2019-SC7-cds82

draG

-

-2.37

-

ADP-ribosylglycohydrolase

  1. aRNA-Seq was used to determine the expression levels of the genes in Hfx. alexandrinus DSM 27206, as detailed in the section on Materials and Methods. The data are the average of three replicates. The threshold of differential expression genes was: |log2(FoldChange)|> 1 and qvalue < 0.00. Dashes indicate that there were minimal changes to the transcription, not considered statistically significant. bThe gene name, number, and annotation were generated through the gDNA sequencing of the tested strain, as described in the Materials and Methods section