Fig. 5

Inhibitory activity of 6xHis-Hill_BB_C10074 against P. aeruginosa PaO1. A Changes in OD measurements of P. aeruginosa PaO1 populations over 16-hours following exposure to either 6xHis-Hill_BB_C10074 (500 µg/mL), Growth control or Buffer control (50mM TrisHCl pH 8.0). Error bars represent standard deviation of the mean. Simple linear regression curves were fitted to the log-growth phase (determined through OD measurements to be between 4–12-hours) to analyse the effect of 6xHis-Hill_BB_C10074 (500 µg/mL) on growth rate during this stage of bacterial growth. Growth rates (k) were calculated as the change in OD over time. Error bars represent the 95% CI. B The number of CFUs in populations of P. aeruginosa PaO1 were calculated periodically over the course of the 16-hour assay.  C P. aeruginosa PaO1 populations were passaged twice against 6xHis-Hill_BB_C10074 and remained susceptible to the antimicrobial activity of the protein as measured by changes in ODs and the number of viable cells (Log₁₀CFU/mL) over 8-hours incubation. All data was inclusive of 3 biological repeats and error bars represent ± standard deviation. Statistics were performed using an unpaired Students’ t-test: ns (not significant) p > 0.05, ** p ≤ 0.01, **** p ≤ 0.0001