Table 2 Sequences of PCR primers for Virulence genes
Genes | Sequence (5ʹ-3ʹ) | Product (bps) | TM |
|---|---|---|---|
agr I | GTCACAAGTACTATAAGCTGCGAT | 440 | 55 |
agr II | GTATTACTAATTGAAAAGTGCCATAGC | 572 | Â |
agr III | CTGTTGAAAAAGTCAACTAAAAGCTC | 406 | Â |
agr IV | CGATAATGCCGTAATACCCG | 588 | Â |
pan-agr | ATGCACATGGTGCACATGC | Â | Â |
sea | GAAAAAAGTCTGAATTGCAGGGAACA | 560 | 55 |
| Â | CAAATAAATCGTAATTAACCGAAGGTTC | Â | Â |
seb | GTCAACCAGATCCTAAACCA | 416 | 54 |
| Â | ACCATCTTCAAATACCCGAA | Â | Â |
sec | AATGGCAATCCTAAACCAGA | 605 | 55 |
| Â | TCAGGCATCAAATCATACCA | Â | Â |
sed | CCAATAATAGGAGAAAATAAAAG | 278 | 60 |
| Â | ATTGGTATTTTTTTTCGTTC | Â | Â |
see | AGGTTTTTTCACAGGTCATCC | 209 | 60 |
| Â | CTTTTTTTTCTTCGGTCAATC | Â | Â |
luk-PV | GGAAACATTTATTCTGGCTATAC | 502 | 50 |
| Â | CTGGATTGAAGTTACCTCTGG | Â | Â |
tsst | ACCCCTGTTCCCTTATCATC | 326 | 60 |
| Â | TTTTCAGTATTTGTAACGCC | Â | Â |
SasX | AGAATTAGAAGTACGTCTAAATGC | 615 | 55 |
| Â | GCTGATTATGTAAATGACTCAAATG | Â | Â |