Table 1 Forward and reverse primers sequences used in this study

Pho A-f

pho

F: 5′- GTGACAAAAGCCACACCATAAATGCCT-3′

903

56

[29]

Pho A-r

R: 3′-TACACTGTCATTACGTTGCGGATTTGGCGT-5′

Ecoli-f

Ecoli

F: 5′-TGACGTTACCCGCAGAAGAA-3′

832

55

[31]

Ecoli-r

R: 3′-CTCCAATCCGGACTACGACG-5′

mcr-1_mp_f

mcr-1

F: 5′- AGTCCGTTTGTTCTTGTGGC-3′

320

58

[33]

mcr-1_mp_r

R: 3′- AGATCCTTGGTCTCGGCTTG-5′

mcr-2_mp_f

mcr-2

F: 5′- CAAGTGTGTTGGTCGCAGTT-3′

715

mcr-2_mp_r

R: 3′- TCTAGCCCGACAAGCATACC-5′

mcr-3_mp_f

mcr-3

F: 5′- AAATAAAAATTGTTCCGCTTATG-3′

929

mcr-3_mp_r

R:3′- AATGGAGATCCCCGTTTTT-5′

mcr-4_mp_f

mcr-4

F:5′-TCACTTTCATCACTGCGTTG-3′

1116

mcr-4_mp_r

R:3′-TTGGTCCATGACTACCAATG-5′

mcr-5_mp_f

mcr-5

F:5′-ATGCGGTTGTCTGCATTTATC-3′

1644

mcr-5_mp_r

R:3′-TCATTGTGGTTGTCCTTTTCTG-5′

mcr-6_mp_f

mcr-6

F:5′-AGCTATGTCAATCCCGTGAT-3′

252

55

[34]

mcr-6_mp_r

R:3′-ATTGGCTAGGTTGTCAATC-5′

mcr-7_mp_f

mcr-7

F:5′-GCCCTTCTTTTCGTTGTT-3′

551

mcr-7_mp_r

R:3′-GGTTGGTCTCTTTCTCGT-5′

mcr-8_mp_f

mcr-8

F:5′-TCAACAATTCTACAAAGCGTG-3′

856

mcr-8_mp_r

R:3′-AATGCTGCGCGAATGAAG-5′

mcr-9_mp_f

mcr-9

F:5′-TTCCCTTTGTTCTGGTTG-3′

1011

mcr-9_mp_r

R:3′-GCAGGTAATAAGTCGGTC-5′

ChuA. 1b

chu A

F:5′-TGCCATCAACACAGTATATCC-3′

288

59

[35]

ChuA.2

R:3′-TCAGGTCGCGAGTGACGGC-5′

YjaA.1b

yja A

F:5′-ATCACATAGGATTCTGCCG-3′

211

YjaA.2b

R:3′-CAGCGGAGTATAGATGCCA-5′

TspE4C2.1b

TspE4.C2

F:5′-AAGGATTCGCTGTTACCGGAC-3′

152

TspE4C2.2b

R:3′-AACTCCTGATACAGGTGGC-5′

AceK.f

arpA

F:5′-TGATATCACGCAGTCAGTAGC-3′

400

ArpA1.r

R:3′-CCGGCCATATTCACATAA-5′

trpAgpC.1

trpAgpC

F:5′-ACAAAAAGTTCTATCGCTTCC-3′

219

62

trpAgpC.2

R:3′-CCTGATCCAGATGATGCTC-5′

ArpAgpE.f

ArpAgpE

F:5′-ACTATTCTCTGCAGGAAGTC -3′

301

59

ArpAgpE.r

R:3′-CTTCCGATGTTCTGAACGT-5′

trpBA.f

trpBA

F:5′-TCCTGGGACATAATGGTCAG-3′

489

trpBA.r

R:3′-GTGTCAGAACGGAATTGT-5′