Table 2 Number of isolates for each species depending on the treatment method and used medium

Agar

WSP

BHI

WSP

BHI

WSP

BHI

Family/Species

Numbers of isolates (% representation from the total isolate number per used method and cultivation medium)

Clostridiaceae

C. baratii

nd

nd

1 (0.9%)

nd

1 (0.7%)

nd

C. butyricum

4 (2.4%)

3 (2%)

3 (2.7%)

2 (1.8%)

5 (3.6%)

11 (8.5%)

C. disporicum

nd

nd

1 (0.9%)

nd

1 (0.7%)

3 (2.3%)

C. jeddahense

nd

nd

2 (1.8%)

3 (2.7%)

nd

nd

C. paraputrificum

13 (7.7%)

12 (8%)

6 (5.3%)

2 (1.8%)

21 (15.3%)

12 (9.3%)

C. perfringens

23 (13.6%)

14 (9.3%)

17 (15%)

12 (10.8%)

27 (19.7%)

20 (15.5%)

C. ramosum

5 (3%)

nd

nd

2 (1.8%)

10 (7.3%)

4 (3.1%)

C. tertium

14 (8.3%)

5 (3.3%)

10 (8.8%)

7 (6.3%)

8 (5.8%)

8 (6.2%)

P. bifermentans

nd

nd

3 (2.7%)

nd

nd

nd

Lachnospiraceae

C. symbiosum

11 (6.5%)

10 (6.7%)

nd

nd

6 (4.4%)

13 (10.1%)

Ei. tayi

nd

nd

nd

nd

2 (1.5%)

2 (1.6%)

En. aldenensis

nd

nd

nd

nd

nd

1 (0.8%)

En. bolteae

nd

nd

nd

nd

1 (0.7%)

nd

R. gnavus

5 (3%)

11 (7.3%)

2 (1.8%)

2 (1.8%)

1 (0.7%)

1 (0.8%)

Oscillospiraceae

F. plautii

1 (0.6%)

1 (0.7%)

nd

nd

nd

1 (0.8%)

Peptostreptococcaceae

Cl. difficile

2 (1.2%)

4 (2.7%)

1 (0.9%)

1 (0.9%)

5 (3.6%)

6 (4.7%)

E. tenue

6 (3.6%)

8 (5.3%)

10 (8.8%)

18 (16.2%)

2 (1.5%)

3 (2.3%)

I. bartlettii

3 (1.8%)

nd

nd

nd

nd

1 (0.8%)

P. sordellii

nd

nd

2 (1.8%)

1 (0.9%)

1 (0.7%)

1 (0.8%)

Total of Eubacteriales isolates

87 (51.5%)

68 (45.3%)

58 (51.3%)

50 (45%)

91 (66.4%)

87 (67.4%)

Detected species/medium

Detected species/treatment

11/21

9/21

12/21

10/21

14/21

15/21

11/21

13/21

17/21

Other detected taxa*

16 (9.5%)

16 (10.7%)

8 (7.1%)

6 (5.4%)

5 (3.6%)

5 (3.9%)

Co-cultures

23 (13.6%)

33 (22%)

20 (17.7%)

18 (16.2%)

31 (22.6%)

27 (20.9%)

Unidentified^‡

43 (25.4%)

33 (22%)

27 (23.9%)

37 (33.3%)

10 (7.3%)

10 (7.8%)

Total isolates (100%)

169

150

113

111

137

129