Table 1 Oligonucleotides employed for RT-RPA and crRNA in RT-RPA-Cas12a-mediated assay for rhinovirus species B detection
From: Establishment of RT-RPA-Cas12a assay for rapid and sensitive detection of human rhinovirus B
Assay | Name | Oligonucleotide sequences (5’-3’) |
|---|---|---|
RT-RPA | HRV-B-F1 | AYTAGTYTGGTCGATGAGGCT |
HRV-B-F2 | AGCCTGCGTGGCGGCCARCCCAGC | |
HRV-B-F3 | ATGTGCTTGRTTGWGANTCCTCCGGCCCCTGAATG | |
HRV-B-R1 | ATATGCTGTGACCATAAGAMAA | |
HRV-B-R2 | CGGACACCCAAAGTAGTCGGTCCCRTCCCRGAATT | |
HRV-B-R3 | GAAACACGGACACCCAAAGTAGTCGGTCCCRTCCC | |
HRV-B-R4 | GTWGANACYTGHGCDCCCATGRTCACAGTATAT | |
RT-RPA-Cas12-based detection | HRV-B-crRNA1-F | GAAATTAATACGACTCACTATAGGGTAATTTCTACTAAGTGTAGATTCCTCCGGCCCCTGAATGCG |
HRV-B-crRNA1-R | CGCATTCAGGGGCCGGAGGAATCTACACTTAGTAGAAATTACCCTATAGTGAGTCGTATTAATTTC | |
HRV-B-crRNA2-F | GAAATTAATACGACTCACTATAGGGTAATTTCTACTAAGTGTAGATACCGACTACTTTGGGTGTCC | |
HRV-B-crRNA2-R | GGACACCCAAAGTAGTCGGTATCTACACTTAGTAGAAATTACCCTATAGTGAGTCGTATTAATTTC | |
HRV-B-crRNA2r-F | GAAATTAATACGACTCACTATAGGGTAATTTCTACTAAGTGTAGATGGACACCCAAAGTAGTCGGT | |
HRV-B-crRNA2r-R | ACCGACTACTTTGGGTGTCCATCTACACTTAGTAGAAATTACCCTATAGTGAGTCGTATTAATTTC | |
HRV-B-crRNA3-F | GAAATTAATACGACTCACTATAGGGTAATTTCTACTAAGTGTAGATACTTTGGGTGTCCGTGTTTC | |
HRV-B-crRNA3-R | GAAACACGGACACCCAAAGTATCTACACTTAGTAGAAATTACCCTATAGTGAGTCGTATTAATTTC | |
HRV-B-crRNA3r-F | GAAATTAATACGACTCACTATAGGGTAATTTCTACTAAGTGTAGATGAAACACGGACACCCAAAGT | |
HRV-B-crRNA3r-R | ACTTTGGGTGTCCGTGTTTCATCTACACTTAGTAGAAATTACCCTATAGTGAGTCGTATTAATTTC | |
ssDNA-reporter-FQ | 6-FAM-TTATTATT-BHQ1 |