Fig. 4
From: Establishment of RT-RPA-Cas12a assay for rapid and sensitive detection of human rhinovirus B
Specificity evaluation of RT-RPA-Cas12a-mediated fluorescence assay using RNA from viral samples, including human rhinoviruses B (HRV-B) species, the mixture of human rhinoviruses A and C species (HRV-AC) in equal volume, metapneumovirus (HmPV), adenovirus (ADV), bocavirus (HBOV), and respiratory syncytial virus (RSV), as the template of RT-RPA, respectively. (A) Specificity evaluation of RT-RPA-Cas12a-mediated real-time fluorescence assay with a fluorescence detector readout. (B) Specificity examination of the RPA-Cas12a-mediated end-point fluorescence assay at 15 min. The data are presented as mean ± SD (n = 3). NC, negative control group