Fig. 1
From: Establishment of RT-RPA-Cas12a assay for rapid and sensitive detection of human rhinovirus B
Schematic diagrams of RT-RPA-Cas12a-mediated fluorescence assay for the detection of human rhinovirus B (HRV-B). (A) Sequence alignment of available twenty whole genomes of HRV-B. The conserved nucleotide sequence in each position are presented by asterisks. Coloring of the nucleotide sequence was made according to the same pattern presented in MEGA7 software. (B) A schematic illustration different crRNA spacer sequence sites within the target VP4 gene sequence of HRV-B species genome. crRNA spacer sequences are indicated by blue colored text, respectively. RT-RPA: reverse-transcription recombinase polymerase amplification assay