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Fig. 5 | BMC Microbiology

Fig. 5

From: Genome-wide identification of resistance genes and cellular analysis of key gene knockout strain under 5-hydroxymethylfurfural stress in Saccharomyces cerevisiae

Fig. 5

Reactive oxygen species accumulation in S. cerevisiae. (A) Accumulation of reactive oxygen species in cells. (B) The proportion of cells at 0 mM HMF that contained reactive oxygen species after treatment for 0 and 3 h. 2′7'-DCF diacetate (top column): reactive oxygen species indicator dye. DIC (down column): differential interference microscope. Negative: no signal; Positive: there is a signal. *** p < 0.001 indicates significant differences. The data represent aver-ages of three experiments. At least 100 cells were examined on each bright-field image(Figure S2, S3)

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