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Table 6 Log 16S rRNA gene copy numbers of specific bacterial groups for the different experimental conditions of modelled cecal microbiota F3, enumerated by qPCR

From: Glycerol and reuterin-producing Limosilactobacillus reuteri enhance butyrate production and inhibit Enterobacteriaceae in broiler chicken cecal microbiota PolyFermS model

 

Log10 16S rRNA gene copies of taxon/mL (mean ± SD)

Total bacteria

Firmicutes

Ruminococcaceae

Lactobacillus-Leuconostoc-Pediococcus spp.

Bacteroidetes

Enterobacteriaceae

Bifidobacteriaceae

L. reuteri

PTA5_F13

CR

 Pre-treatment

11.3 ± 0.0

10.5 ± 0.1

10.6 ± 0.1

6.9 ± 0.1

5.9 ± 0.2

9.4 ± 0.4

4.9 ± 0.0

BDL

 Treatment

11.2 ± 0.1

10.4 ± 0.1

10.1 ± 0.1

7.5 ± 0.1

5.6 ± 0.0

8.7 ± 0.1

4.7 ± 0.1

BDL

 P value

0.413

0.124

0.005

0.003

0.039

0.007

0.005

-

100G

 Pre-treatment

11.2 ± 0.1

10.5 ± 0.1

10.2 ± 0.1

6.5 ± 0.2

5.7 ± 0.1

9.2 ± 0.0

4.9 ± 0.1

BDL

 Treatment

11.2 ± 0.1

10.5 ± 0.2

10.2 ± 0.2

6.2 ± 0.1

6.7 ± 0.1

8.9 ± 0.3

4.9 ± 0.0

BDL

 P value

0.529

0.800

0.937

0.186

0.001

0.235

0.644

-

Lbr

 Pre-treatment

11.1 ± 0.2

10.5 ± 0.0

10.2 ± 0.1

6.6 ± 0.5

5.5 ± 0.2

9.4 ± 0.7

4.8 ± 0.3

BDL

 Treatment

11.1 ± 0.1

10.4 ± 0.1

10.2 ± 0.0

6.3 ± 0.2

5.6 ± 0.1

9.8 ± 0.2

5.0 ± 0.1

5.1 ± 0.5

 P value

0.923

0.080

0.462

0.369

0.836

0.463

0.349

0.001

Lbr-100G

 Pre-treatment

11.2 ± 0.3

10.5 ± 0.2

10.4 ± 0.1

6.8 ± 0.6

6.0 ± 0.0

10.2 ± 0.3

4.9 ± 0.1

BDL

 Treatment

11.2 ± 0.1

10.6 ± 0.1

10.3 ± 0.2

8.0 ± 0.3

6.4 ± 0.1

9.4 ± 0.3

4.8 ± 0.1

6.1 ± 0.9

 P value

0.825

0.583

0.766

0.052

0.002

0.052

0.644

0.002

  1. Data are means ± SD for the last 3 days of each treatment period. P value is calculated for the pairwise comparison of the last 3 days of treatment and pre-treatment periods within a reactor by unpaired t-test. BDL, below the detection limit (4.8 log gene copies, L. reuteri PTA5_F13). CR, Control reactor; 100G, 100 mM glycerol; Lbr, L. reuteri PTA5_F13; Lbr-100G, L. reuteri PTA5_F13 and 100 mM glycerol