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Table 5 Log 16S rRNA gene copy numbers of specific bacterial groups for the different experimental conditions of modelled cecal microbiota F2, enumerated by qPCR

From: Glycerol and reuterin-producing Limosilactobacillus reuteri enhance butyrate production and inhibit Enterobacteriaceae in broiler chicken cecal microbiota PolyFermS model

 

Log10 16S rRNA gene copies of taxon/mL (mean ± SD)

Total bacteria

Firmicutes

Ruminococcaceae

Lactobacillus-Leuconostoc-Pediococcus spp.

Bacteroidetes

Enterobacteriaceae

Bifidobacteriaceae

L. reuteri

PTA5_F13

CR

 Pre-treatment

10.7 ± 0.0

9.9 ± 0.1

9.8 ± 0.0

6.4 ± 0.1

9.9 ± 0.1

8.5 ± 0.3

7.2 ± 0.1

BDL

 Treatment

10.6 ± 0.1

9.8 ± 0.1

9.8 ± 0.1

6.1 ± 0.0

9.9 ± 0.0

8.2 ± 0.2

7.2 ± 0.1

BDL

 P value

0.116

0.251

0.374

 < 0.001

0.116

0.284

0.230

-

100G

 Pre-treatment

10.8 ± 0.1

9.9 ± 0.1

9.9 ± 0.1

7.8 ± 0.2

8.9 ± 0.1

8.1 ± 0.1

8.4 ± 0.2

BDL

 Treatment

10.8 ± 0.1

10.0 ± 0.0

10.0 ± 0.0

8.1 ± 0.3

8.5 ± 0.2

7.4 ± 0.2

8.3 ± 0.1

BDL

 P value

0.519

0.116

0.116

0.187

0.013

0.003

0.348

-

Lbr

 Pre-treatment

10.9 ± 0.1

9.8 ± 0.0

9.8 ± 0.1

7.3 ± 0.3

9.5 ± 0.2

7.4 ± 0.1

7.7 ± 0.2

BDL

 Treatment

10.8 ± 0.3

9.9 ± 0.1

9.9 ± 0.0

8.9 ± 0.1

9.2 ± 0.1

7.3 ± 0.2

8.2 ± 0.1

7.6 ± 0.1

 P value

0.442

0.007

0.116

 < 0.001

0.193

0.435

0.010

 < 0.001

Lbr-100G

 Pre-treatment

11.0 ± 0.1

9.8 ± 0.0

9.8 ± 0.1

6.4 ± 0.2

10.0 ± 0.1

8.1 ± 0.1

7.3 ± 0.0

BDL

 Treatment

11.1 ± 0.1

9.8 ± 0.1

9.8 ± 0.1

8.9 ± 0.1

9.9 ± 0.1

6.9 ± 0.1

7.2 ± 0.1

7.7 ± 0.1

 P value

0.374

0.374

0.230

 < 0.001

0.319

 < 0.001

0.116

 < 0.001

  1. Data are means ± SD for the last 3 days of each treatment period. P value is calculated for the pairwise comparison of the last 3 days of treatment and pre-treatment periods within a reactor by unpaired t-test. BDL, below the detection limit (4.8 log gene copies, L. reuteri PTA5_F13). CR, Control reactor; 100G, 100 mM glycerol; Lbr, L. reuteri PTA5_F13; Lbr-100G, L. reuteri PTA5_F13 and 100 mM glycerol